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| Reactivity: | H,M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | CD27 antigen |
Synonyms: | CD27, S152, S152. LPFS2, T14, TNFRSF7, Tp55, CD27 molecule |
Immunogen: | Recombinant protein corresponding to Mouse CD27 |
Uniprot ID: | P41272 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Membrane |
Predicted MW. / Observed MW. | 28 kDa / 55 kDa (Human) 50KDa (Mouse) |
Product Usage Information
WB | Human, Mouse | 1: 500-1: 1000 | thymus |
IHC/IF | Mouse, Rat | 1: 1000-1: 2000 | lymph node, spleen |
Background
CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity. It is a receptor for CD70 (Cd27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway. CD27 may also play a role in apoptosis through association with SIVA1.
Images
| Western blot analysis of CD27 (GB111165) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded mouse lymph node using CD27 (GB111165) at dilution of 1:2000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse spleen using CD27 (GB111165) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat lymph node using CD27 (GB111165) at dilution of 1:2000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat spleen using CD27 (GB111165) at dilution of 1:1000 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
