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Recombinant Anti-CD3 Rabbit mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | T-cell surface glycoprotein CD3 epsilon chain |
Synonyms: | CD3E, IMD18, T3E, TCRE, CD3e molecule |
Immunogen: | Recombinant protein corresponding to Human CD3 |
Uniprot ID: | P07766 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 23 kDa / 23 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | Jurket, thymus, Molt-4 |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | stomach, spleen |
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Images
| WB analysis of CD3(GB150004). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of CD3(GB150004). Sample: Human stomach, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD3(GB150004). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD3(GB150004). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD3(GB150004). Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
