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| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Receptor-type tyrosine-protein phosphatase C |
Synonyms: | B220, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, Ly-5, Lymphocyte antigen 5 |
Immunogen: | Recombinant protein corresponding to Human CD45 |
Uniprot ID: | P08575 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 147kDa / 180-250 kDa |
Product Usage Information
WB | Human | 1: 300-1: 600 | Jurkat, THP-1 |
IHC/IF | Human | 1: 300-1: 600 | liver, lymph node |
Background
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).; (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Images
| Western blot analysis of CD45 (GB115428) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human lymph node using CD45 (GB115428) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded human liver using CD45 (GB115428) at dilution of 1: 300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
