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| Reactivity: | M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | T-cell surface glycoprotein CD5 |
Synonyms: | Lymphocyte antigen 1, Ly-1, Lyt-1, CD5, T1, LEU1, LyA, Lymphocyte antigen T1/Leu-1 |
Immunogen: | Recombinant protein corresponding to Mouse CD5 |
Uniprot ID: | P13379 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 54 kDa / 65 kDa |
Applications
WB | Mouse | 1: 500-1: 1000 | spleen, thymus, lymph node |
IHC/IF | Mouse, Rat | 1: 850-1: 3400 | lymph, spleen |
Background
CD5 is a cell surface glycoprotein expressed on a majority of thymocytes, mature T cells and a subset of B cells called B-1 cells. CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development.
Images
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Western blot analysis of CD5 (GB111360) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse spleen using CD5 (GB111360) at dilution of 1: 850 |
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Immunofluorescence analysis of paraffin embedded mouse lymph using CD5 (GB111360) at dilution of 1: 1700 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
