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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CD74 rabbit polyclonal antibody |
Gene Name: | H-2 class II histocompatibility antigen gamma chain |
Synonyms: | Ia antigen-associated invariant chain, Ii, MHC class II-associated invariant chain, CD74 |
Immunogen: | Recombinant protein corresponding to Mouse CD74 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell Membrane |
Uniprot ID: | P04441, P10247 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 500-1: 2000 | colon, lung, lymph node, spleen, thymus |
Background
CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized.
Images
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Immunohistochemistry of paraffin embedded mouse lung using MHC-II (GB111179) at dilution of 1:1800 (400x lens) |
 | Immunohistochemistry of paraffin embedded mouse lymph node using MHC-II (GB111179) at dilution of 1:1800 (400x lens) |
 | Immunohistochemistry of paraffin embedded mouse spleen using MHC-II (GB111179) at dilution of 1:1800 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat lymph node using MHC-II (GB111179) at dilution of 1:1800 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat thymus using MHC-II (GB111179) at dilution of 1:1800 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse lung using MHC-II (GB111179) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded rat colon using MHC-II (GB111179) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded rat lymph node using MHC-II (GB111179) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded rat thymus using MHC-II (GB111179) at dilution of 1:500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
