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| Reactivity: | H,M,R & H |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cyclin-dependent kinase 1 |
Synonyms: | CDK1, CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1, cyclin dependent kinase 1 |
Immunogen: | Recombinant protein corresponding to Mouse CDK1 |
Uniprot ID: | P11440 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 34 kDa / 34 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 2000 | spleen, thymus |
ICC/IF | Human | 1: 100-1: 400 | HeLa |
Background
Cyclin-dependent kinase 1 also known as CDK1 or cell division cycle protein 2 homolog is a highly conserved protein that functions as a serine/threonine kinase, and is a key player in cell cycle regulation. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Images
|
Western blot analysis of cdc2 (GB11635) at dilution of 1: 500 |
| ICC analysis of CDK1(GB11635)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
