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| Reactivity: | H,M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cyclin-dependent kinase 6 |
Synonyms: | Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6, MCPH12, MGC59692, PLSTIRE, STQTL11, Crk2, Cell division protein kinase 6 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human CDK6 |
Uniprot ID: | Q00534 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 37 kDa / 37 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus, testis |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
Cyclin-dependent kinase 6 (p40cdk6 also known as PLSTIRE) belongs to a family of proteins designated as cdks. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).
Images
| WB analysis of CDK6(GB111607). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
|
ICC analysis of CDK6(GB111607)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
