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Anti-CDKN2A/p14ARF Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Tumor suppressor ARF |
Synonyms: | ARF, ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse p14ARF |
Uniprot ID: | Q64364 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Mitochondrion |
Product Usage Information
IHC/IF | Human | 1: 150-1: 600 | lung, liver, tonsil |
Background
Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform smARF may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.
Images
| Immunohistochemistry analysis of paraffin embedded human cervical carcinoma using CDKN2A/p14ARF (GB115589) at dilution of 1: 200 |
| Immunohistochemistry analysis of paraffin embedded human cervical carcinoma using CDKN2A/p14ARF (GB115589) at dilution of 1: 200 |
| Immunohistochemistry analysis of paraffin embedded human esophageal cancer using CDKN2A/p14ARF (GB115589) at dilution of 1: 200 |
| Immunohistochemistry analysis of paraffin embedded human liver using CDKN2A/p14ARF (GB115589) at dilution of 1: 200 |
| Immunohistochemistry analysis of paraffin embedded human lung cancer using CDKN2A/p14ARF (GB115589) at dilution of 1: 200 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using CDKN2A/p14ARF (GB115589) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
