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Anti-CENPF Rabbit pAb (100 μl)
| Reactivity: | H & H,M |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Centromere protein F |
Synonyms: | AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human CENPF |
Uniprot ID: | P49454 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus, Chromosome |
Product Usage Information
IHC/IF | Human | 1: 500-1: 2000 | gastric cancer, colon, tonsil |
ICC/IF | Human, Mouse | 1: 100-1: 600 | NIH-3T3, HeLa |
Background
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
Images
| Immunohistochemistry analysis of paraffin embedded human gastric cancer using CENPF (GB115058) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human colon using CENPF (GB115058) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using CENPF (GB115058) at dilution of 1: 600 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using CENPF (GB115058) at dilution of 1: 400 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using CENPF (GB115058) at dilution of 1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
