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-23%
Anti-CH25H Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CH25H rabbit polyclonal |
Gene Name: | Cholesterol 25-hydroxylase |
Synonyms: | Cholesterol 25-monooxygenase, m25OH, Ch25h, C25H |
Uniprot ID: | |
Immunogen: | Recombinant protein corresponding to Mouse CH25H |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 35 kDa |
Observed MW. | 35 kDa |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | U-87MG, HaCaT |
Background
CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
Images
 | Western blot analysis of CH25H (GB113221) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
