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Recombinant Anti-COX IV antibody (HRP Conjugated) (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
Synonyms: | Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a, COX4I1, COX4, COX4-1, COXIV, COXIV-1, COXIV-1, cytochrome c oxidase subunit 4I1 |
Immunogen: | Recombinant protein corresponding to Mouse COX IV |
Uniprot ID: | P19783 |
Isotype: | IgG1 |
Conjugation | HRP |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 20 kDa / 17 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Mouse, Rat | 1: 1000-1: 2000 | brain, liver |
Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-COX IV antibody(ZB15250-HRP) for 3-4 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.
Images
| WB analysis of COX IV(ZB15250-HRP). Antibody: HRP conjugated anti-COX IV antibody 1: 2000, 4°C overnight. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
