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| Reactivity: | H,R & M |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Mast cell carboxypeptidase A |
Synonyms: | Carboxypeptidase A3, MC CPA |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CPA3 |
Uniprot ID: | P15089 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Product Usage Information
IHC/IF | Human, Rat | 1: 800-1: 2400 | colon, esophagus |
ICC/IF | Mouse | 1: 100-1: 400 | NIH-3T3 |
Background
Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.
Images
| Immunohistochemistry analysis of paraffin embedded rat colon using CarboxypeptidaseA3 (GB114968) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded human esophagus using CarboxypeptidaseA3 (GB114968) at dilution of 1: 800 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using CarboxypeptidaseA3 (GB114968) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
