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Anti-CYP24A1 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial |
Synonyms: | CYP24A1, Cyp-24, Cyp24, Cytochrome P450-CC24, Cytochrome P450 24A1 |
Immunogen: | Recombinant protein corresponding to Mouse CYP24A1 |
Uniprot ID: | Q64441 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 59 kDa / 59 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 1000-1: 3000 | liver, skeletal muscle, kidney |
Background
A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24-oxidation pathway, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D3) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D3).
With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. Hydroxylates at C-24 or C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.
Images
 | Western blot analysis of CYP24A1 (GB113873) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
