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Anti-CYP27B1 Rabbit pAb (100 μl)
| Reactivity: | M & H,M,R & M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial |
Synonyms: | Cyp27b, Cyp40, Cytochrome p450 27B1, Cytochrome P450VD1-alpha, VD3 1A hydroxylase, VDDR, VDD1, VDDRI, VDR, PDDR, CYP1, CP2B, CYP1ALPHA |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CYP27B1 |
Uniprot ID: | O35084 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion membrane |
Predicted MW. / Observed MW. | 56 kDa / 56 kDa |
Product Usage Information
WB | Mouse | 1: 2000-1: 4000 | kidney |
IHC/IF | Human, Mouse, Rat | 1: 800-1: 2400 | pancreas, kidney |
ICC/IF | Mouse | 1: 100-1: 400 | NIH-3T3 |
Background
A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway.
Images
| Western blot analysis of CYP27B1 (GB114918) at dilution of 1: 2000 |
| Immunohistochemistry of paraffin embedded rat pancreas using CYP27B1 (GB114918) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded mouse kidney using CYP27B1 (GB114918) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded human kidney using CYP27B1 (GB114918) at dilution of 1: 800 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using CYP27B1 (GB114918) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
