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Anti-Caprin-1 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Caprin-1 |
Synonyms: | caprin 1, CAPRIN1, GPI anchored protein p137, GPI-p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105, G5E5, Gpiap, ytoplasmic activation- and proliferation-associated protein 1 |
Immunogen: | Recombinant protein corresponding to Mouse Caprin-1 |
Uniprot ID: | Q60865 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 78 kDa / 116 kDa |
Product Usage Information
WB | Human, Mouse | 1: 1000-1: 1500 | testis |
ICC/IF | Human, Mouse | 1: 100-1: 300 | HeLa |
Background
CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.
Images
| WB analysis of Caprin-1(GB113530). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of Caprin-1(GB113530)green. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
