Recombinant Anti-Carbonic Anhydrase 9/CA9 Rabbit mAb (100 μl)

Recombinant Anti-Carbonic Anhydrase 9/CA9 Rabbit mAb (100 μl)

Brand: ServiceBio
Cat. #: GB153899-100
Availability: In Stock
$259.00
-+
Reactivity: H    
Applications: IHC/IF    
Host Species: Rabbit    
Clonality: Monoclonal    
Gene Name:

Carbonic anhydrase 9

Synonyms:

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

Immunogen:

Recombinant protein corresponding to Human CA9

Uniprot ID:

Q16790

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Nucleus, Cell membrane, Cell projection




Product Usage Information

IHC/IF

Human

1: 500-1: 1000

gastric carcinoma, colon cancer




Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known.




Images

01 GB153899_Carbonic Anhydrase 9 CA9_R22132_(A367 A368)_d1000_H colon cancer_40x_IHC._WM.png


IHC analysis of Carbonic Anhydrase 9/CA9(GB153899).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02 GB153899_Carbonic Anhydrase 9 CA9_R22132_(A367 A368)_d1000_H gastric carcinoma_23.7x_IHC_WM.png

IHC analysis of Carbonic Anhydrase 9/CA9(GB153899).
Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage

Storage            Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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