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Anti-Cardiac Troponin I Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cardiac Troponin I |
Synonyms: | Cardiac troponin I, TNNI3, TNNC1, cTnI, CMH7, cardiac muscle, TNNI3, Troponin I type 3 cardiac , cardiac type, CTnI |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Cardiac Troponin I |
Uniprot ID: | P19429 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. / Observed MW. | 24 kDa / 28 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 250-1: 500 | heart |
IHC/IF | Mouse, Rat | 1: 100-1: 400 | heart, myocardial infarction |
Background
Troponin I is a cardiac and skeletal muscle protein useful in the laboratory diagnosis of heart attack. Troponin I is a part of the troponin protein complex, where it binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
Images
| Western blot analysis of cTnI (GB112408) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded mouse heart using CTNI (GB112408) at dilution of 1: 200 |
| Immunohistochemistry analysis of paraffin embedded rat myocardial infarction using CTNI (GB112408) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
