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Recombinant Anti-Caspase-1 Rabbit mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Caspase-1 |
Synonyms: | CASP1, ICE, IL1BC, P45, caspase 1 |
Immunogen: | Recombinant protein corresponding to Human caspase1 |
Uniprot ID: | P29466 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 45 kDa / 45-50 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | THP-1, spleen, thymus |
IHC/IF | Human, Mouse, Rat | 1: 750-1: 3000 | colon cancer, spleen |
Background
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Images
| WB analysis of Caspase-1(GB15383). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of Caspase-1(GB15383). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Caspase-1(GB15383). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Caspase-1(GB15383). Sample: Mouse lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Caspase-1(GB15383). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Caspase-1(GB15383). Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Caspase-1(GB15383). Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
