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Anti-Caspase-3 Rabbit pAb (100 μl)
| Reactivity: | H,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Caspase-3 rabbit polyclonal |
Gene Name: | Caspase-3 |
Synonyms: | Casp3, CASP3, CPP32, CPP32B, SCA-1, Caspase 3 |
Immunogen: | Recombinant protein corresponding to Mouse Caspase3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Uniprot ID: | P42574, P70677, P55213 |
Product Usage Information
IHC/IF | Human, Rat | 1: 100-1: 200 | breast cancer, liver cancer, colon cancer |
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Images
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IHC analysis of Caspase-3 (GB11009-1). Sample: Human breast cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of Caspase-3 (GB11009-1). Sample: Human liver cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
|
IHC analysis of Caspase-3 (GB11009-1). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
