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Anti-Caspase 7 Mouse mAb (100 μl)
| Reactivity: | H & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Caspase-7 |
Synonyms: | Apoptotic protease Mch 3, CASP-7, CASP7, Caspase 7, Caspase7, CMH-1, ICE-LAP3, ICE like apoptotic protease 3, MCH3 |
Immunogen: | Recombinant protein corresponding to Human Caspase 7 |
Uniprot ID: | P55210 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 34 kDa / 20,/30/35 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | Jurkat |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 3000 | appendix, colon, ileum |
Background
Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury. The protein is involved in the activation cascade of caspases responsible for apoptosis execution.
Images
| Western blot analysis of CASPASE7 (GB12540) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human appendix using caspase7 (GB12540) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse colon using caspase7 (GB12540) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin embedded rat ileum using caspase7 (GB12540) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
