Reactivity: | H |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Cdc27 rabbit polyclonal antibody |
Gene Name: | Cell division cycle protein 27 homolog |
Synonyms: | Cdc27, ANAPC3, APC3, Nuc2 homolog, HNUC, Cell Division Cycle 27, Anaphase-promoting complex subunit 3 |
Immunogen: | Recombinant protein corresponding to Mouse Cdc27 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 92 kDa |
Observed MW. | 92 kDa |
Uniprot ID: | P30260 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | K562, HeLa, Jurkat |
Background
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins.
Images
Western blot analysis of CDC27 (GB111996) at dilution of 1: 500 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |