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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Chk2 rabbit polyclonal antibody |
Gene Name: | Serine/threonine-protein kinase Chk2 |
Synonyms: | CHEK2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Checkpoint kinase 2 |
Immunogen: | Recombinant protein corresponding to Mouse Chk2 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 61 kDa |
Observed MW. | 61 kDa |
Uniprot ID: | O96017, Q9Z265 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, colon, thymus |
Background
CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2, a serine-threonine kinase. CHK2 regulates cell division, and has the ability to prevent cells from dividing too rapidly or in an uncontrolled manner, So CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2. Once activated, CHK2 phosphorylates downstream targets including CDC25 phosphatases, responsible for dephosphorylating and activating the cyclin-dependent kinases (CDKs). Thus, CHK2’ s inhibition of the CDC25 phosphatases prevents entry of the cell into mitosis.
Images
 |
Western blot analysis of CHK2 (GB11574) at dilution of 1: 500 |
Storage
| Storage |
Store at -20°C for one year. Avoid repeated freeze/thaw cycles |
| Storage Buffer |
PBS with 0.02%sodium azide, 100 μg/ml BSA and 50% glycerol. |
