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Anti-Choline Acetyltransferase Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Choline Acetyltransferase rabbit polyclonal antibody |
Gene Name: | Choline O-acetyltransferase |
Synonyms: | CHAT, CHOACTASE, CMS1A, CMS1A2, CMS6, choline O-acetyltransferase |
Immunogen: | Recombinant protein corresponding to Mouse ChAT |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytosol, Nucleus, Presynapse |
Uniprot ID: | Q03059, P32738 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 1000-1: 2000 | brain, spinal cord |
Background
Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. ChAT catalyzes the transfer of an acetyl group from the coenzyme acetyl-CoA to choline, yielding acetylcholine (ACh). ChAT is found in high concentration in cholinergic neurons, both in the central nervous system (CNS) and peripheral nervous system (PNS). As with most nerve terminal proteins, ChAT is produced in the body of the neuron and is transported to the nerve terminal, where its concentration is highest. Presence of ChAT in a nerve cell classifies this cell as a "cholinergic" neuron.
Images
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Immunohistochemistry of paraffin embedded mouse brain using chat (GB11070-1) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using chat (GB11070-1) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat spinal cord using chat (GB11070-1) at dilution of 1:1000 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
