Recombinant Anti-Collagen II Mouse mAb (100 μl)

Recombinant Anti-Collagen II Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB15021-100
Availability: In Stock
$259.00
-+
Reactivity: M,R    
Applications: IHC/IF    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Collagen alpha-1(II) chain

Synonyms:

COL2A1, ANFH, AOM, COL11A3, SEDC, STL1, collagen type II alpha 1, collagen type II alpha 1 chain

Immunogen:

Recombinant protein corresponding to Mouse collagen II

Uniprot ID:

P28481

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Secreted,Extracellular matrix




Product Usage Information

IHC/IF

Mouse, Rat

1: 500-1: 1000

bone




Background

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.




Images

01 GB15021_CollagenII_ZSM17011_d1200_M bone_20.0x_IHC.png

IHC analysis of CollagenII(GB15021).
Sample: Mouse bone,4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1:1200, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h.

02 GB15021_CollagenII_ZSM17011_d1200_R bone_20.0x_IHC.png

IHC analysis of CollagenII(GB15021).
Sample: Rat bone,4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1:1200, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h.




Storage

Storage            Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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