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Anti-Cubilin Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cubilin |
Synonyms: | Intrinsic factor-cobalamin receptor, Cubn, IFCR, MGA1, megaloblastic anemia 1, intrinsic factor B12-receptor, Cubilin precursor |
Immunogen: | Recombinant protein corresponding to Mouse Cubilin |
Uniprot ID: | Q9JLB4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Endosome, Lysosome |
Predicted MW. / Observed MW. | 399 kDa / 399 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | kidney |
IHC/IF | Human, Mouse, Rat | 1: 650-1: 1300 | kidney, small intestine, duodenum |
Background
Cubilin is an endocytic receptor that lacks a classical transmembrane region. It is a multidomain receptor that contains an amino terminal 110 residue segment followed by 8 epidermal growth factor (EGF)-like repeats and a contiguous stretch of 27 CUB domains. Cubilin acts as a receptor for intrinsic factor-vitamin B12 complexes. Cubilin is located within the epithelium of intestine and kidney. Mutations in Cubilin may play a role in autosomal recessive megaloblastic anemia also known as Imerslund-Grasbeck’s disease, which causes intestinal malabsorption of vitamin B12.
Images
| Western blot analysis of CUBN (GB112071) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraffin embedded rat kidney using CUBN (GB112071) at dilution of 1: 650 |
| Immunofluorescent analysis of paraffin embedded rat duodenum using CUBN (GB112071) at dilution of 1: 650 |
| Immunofluorescent analysis of paraffin embedded mouse small intestine using CUBN (GB112071) at dilution of 1: 650 |
| Immunofluorescent analysis of paraffin embedded human kidney using CUBN (GB112071) at dilution of 1: 650 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
