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Anti-Cyclin B1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Cyclin B1 rabbit polyclonal antibody |
Gene Name: | G2/mitotic-specific cyclin-B1 |
Synonyms: | CCNB, CCNB1, Cyclin B1, G2/mitotic specific cyclin B1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 55 kDa |
Observed MW. | 55 kDa |
Uniprot ID: | P14635, P24860, P30277 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 250-1: 500 | lung |
IHC/IF | Human, Mouse, Rat | 1: 100-1: 800 | brain, lung, testis |
Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Images
| Western blot analysis of CyclinB1 (GB11255) at dilution of 1: 250 |
| Immunohistochemistry of paraffin embedded mouse testis using Cyclin-B1 (GB11255) at dilution of 1: 400 (300x lens) |
| Immunohistochemistry of paraffin embedded rat brain using Cyclin-B1 (GB11255) at dilution of 1: 400 (400x lens) |
| Immunohistochemistry of paraffin embedded rat lung using Cyclin-B1 (GB11255) at dilution of 1: 400 (300x lens) |
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Immunohistochemistry of paraffin embedded rat testis using Cyclin-B1 (GB11255) at dilution of 1: 400 (300x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
