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Anti-Cytochrome C Mouse mAb (100 μl)
| Reactivity: | M,R & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Cytochrome c |
Synonyms: | CYCS, CYC, HCS, THC4, cytochrome c, somatic, Cytochrome c, cyt c |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cytochrome c |
Uniprot ID: | P62897 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion intermembrane space |
Predicted MW. / Observed MW. | 12 kDa / 14 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | heart |
IHC/IF | Human, Rat | 1: 250-1: 1000 | colon, stomach cancer, liver |
Background
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase.Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol.
Images
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Western blot analysis of Cyt-C (GB12080) at dilution of 1: 1000 |
 | Immunohistochemistry of paraffin embedded human stomach cancer using cyt-c (GB12080) at dilution of 1: 2000 (300x lens) |
 | Immunohistochemistry of paraffin embedded mouse heart using cyt-c (GB12080) at dilution of 1: 6000 (380x lens) |
 | Immunohistochemistry of paraffin embedded rat liver using cyt-c (GB12080) at dilution of 1: 2000 (320x lens) |
 | Immunohistochemistry of paraffin embedded rat heart using cyt-c (GB12080) at dilution of 1: 2000 (220x lens) |
 | Immunofluorescence of paraffin embedded human colon using cytc (GB12080) at dilution of 1: 500 (400x lens) |
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Immunofluorescence of paraffin embedded human kidney using cytc (GB12080) at dilution of 1: 500 (400x lens) |
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Immunofluorescence of paraffin embedded rat kidney using cytc (GB12080) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
