Recombinant Anti-Cytokeratin 17 Mouse mAb (100 μl)

Keratin, type I cytoskeletal 17

Synonyms:

Cytokeratin-17, CK-17, Keratin-17, Krt1-17, Krt17, K17, keratin 17 epitope S1, PCHC1, PC2

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human Cytokeratin 17

Uniprot ID:

Q9QWL7

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Cytoplasm

Predicted MW. / Observed MW.

48 kDa / 48 kDa

WB

Human

1: 500-1: 2000

Hela, HaCaT, A431

IHC/IF

Human

1: 500-1: 1000

esophageal carcinoma, Laryngeal squamous cell carcinoma

WB analysis of Cytokeratin 17(GB15363).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.

IHC analysis of Cytokeratin 17(GB15363).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of Cytokeratin 17(GB15363).
Sample: Human Laryngeal squamous cell carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.