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Anti-Cytokeratin 18 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Cytokeratin 18 rabbit polyclonal |
Gene Name: | Keratin, type I cytoskeletal 18 |
Synonyms: | KRT18,CK-18,CK18,CYK18,K18,keratin 18 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cytokeratin 18 |
Isotype: | IgG |
Predicted MW. | 48 kDa |
Observed MW. | 48 kDa |
Purity: | Affinity purification |
Subcellular location: | |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human,Mouse,Rat | 1:500-1:1000 | Colon,A548,HepG2 |
IHC | Human,Mouse,Rat | 1:300-1:1000 | Kidney,stomach |
IF | Human,Mouse,Rat | 1:200-1:1000 | Breast cancer |
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Images
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Western blot analysis of Cytokeratin 18 (GB11232) at dilution of 1:1000. Lane 1:A549 cell lysate Lane 2:HepG2 cell lysate Lane 3:MCF7 cell lysate Lane 4:SW480 cell lysate Lane 5:Mouse colon tissue lysate Lane 6:Mouse colon tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
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Immunohistochemistry analysis of paraffin-embedded human liver using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
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Immunohistochemistry analysis of paraffin-embedded human stomach using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
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Immunohistochemistry analysis of paraffin-embedded human kidney using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse liver using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse kidney using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse stomach using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse uterus using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
| Immunohistochemistry analysis of paraffin-embedded rat lung using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
| Immunohistochemistry analysis of paraffin-embedded rat liver using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
| Immunohistochemistry analysis of paraffin-embedded rat kidney using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
| Immunohistochemistry analysis of paraffin-embedded rat pancreas using Cytokeratin 18 (GB11232) at dilution of 1:1000 |
| Immunofluorescence analysis of paraffin-embedded human breast cancer using Cytokeratin 18 (GB11232) at dilution of 1:500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
