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Anti-Cytokeratin 20 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Keratin, type I cytoskeletal 20 |
Synonyms: | Cytokeratin-20, CK-20, Keratin-20, K20, Krt20, CD20, KA20, Protein IT |
Immunogen: | Recombinant protein corresponding to Mouse Cytokeratin 20 |
Uniprot ID: | Q9D312 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 49 kDa / 49 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | colon, small intestine |
IHC/IF | Human, Mouse, Rat | 1: 1500-1: 3000 | colon, duodenum, rectum, small intestine, ileum, jejunum |
Background
Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.
Images
| Western blot analysis of KERATIN20 (GB112050) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human colon using K20 (GB112050) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat colon using K20 (GB112050) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded mouse duodenum using K20 (GB112050) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded mouse rectum using K20 (GB112050) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat rectum using K20 (GB112050) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat small intestine using K20 (GB112050) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human colon cancer using K20 (GB112050) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human bladder cancer using K20 (GB112050) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human gastric cancer using K20 (GB112050) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
