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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 |
Synonyms: | DAD-1, DAD1, defender against cell death 1, OST2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse DAD1 |
Uniprot ID: | P61804 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 12 kDa / 16 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 100-1: 300 | testis |
Background
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Images
| Western blot analysis of DAD1 (GB114611) at dilution of 1: 300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
