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Anti-DAP13/NDUFA12 Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 |
Synonyms: | B17.2, CI B17.2, CIB17.2, Complex I B17.2, DAP13, NDUFA12, RGD1311462, 13 kDa differentiation associated protein |
Immunogen: | Recombinant protein corresponding to Mouse DAP13/NDUFA12 |
Uniprot ID: | Q7TMF3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 17 kDa / 17 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | skeletal muscle, kidney |
ICC/IF | Human. Mouse | 1: 50-1: 200 | HeLa, NIH-3T3 |
Background
This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of NDUFA12 (GB113175) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using DAP13(GB113175) at dilution of 1: 50-1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
