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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | DDX5 rabbit polyclonal antibody |
Gene Name: | Probable ATP-dependent RNA helicase DDX5 |
Synonyms: | DEAD box RNA helicase DEAD1, mDEAD1, DEAD box protein 5, RNA helicase p68, Ddx5, Tnz2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse DDX5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 69 kDa |
Observed MW. | 69 kDa |
Uniprot ID: | P17844, Q61656 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | testis |
IHC/IF | Mouse, Rat | 1: 500-1: 1500 | bladder, brain, breast, colon, testis |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus.
Images
| Western blot analysis of DDX5 (GB112552) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded mouse brain using DDX5 (GB112552) at dilution of 1: 700 (400x lens) |
| Immunofluorescence of paraffin embedded mouse bladder using DDX5 (GB112552) at dilution of 1: 700 (400x lens) |
| Immunofluorescence of paraffin embedded rat breast using DDX5 (GB112552) at dilution of 1: 700 (400x lens) |
| Immunofluorescence of paraffin embedded rat colon using DDX5 (GB112552) at dilution of 1: 700 (400x lens) |
| Immunofluorescence of paraffin embedded mouse brain using DDX5 (GB112552) at dilution of 1: 700 (250x lens) |
 | Immunofluorescence of paraffin embedded mouse colon using DDX5 (GB112552) at dilution of 1: 700 (200x lens) |
 | Immunofluorescence of paraffin embedded mouse testis using DDX5 (GB112552) at dilution of 1: 700 (250x lens) |
| Immunofluorescence of paraffin embedded rat brain using DDX5 (GB112552) at dilution of 1: 700 (250x lens) |
| Immunofluorescence of paraffin embedded rat testis using DDX5 (GB112552) at dilution of 1: 700 (250x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
