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Anti-DFFB/CAD Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | DFFB/CAD rabbit polyclonal antibody |
Gene Name: | DNA fragmentation factor subunit beta |
Synonyms: | Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, DNA fragmentation factor 40 kDa subunit, DFF-40, Dffb, Cad, CPAN |
Immunogen: | Recombinantproteincorresponding to Mouse DFFB/CAD |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 39 kDa |
Observed MW. | 39 kDa |
Uniprot ID: | O76075, O54788, Q99N34 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, thymus, colon |
Background
Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA. Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs).
Images
| Western blot analysis of DFFB (GB111721) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
