Recombinant Anti-Desmin Rabbit mAb (100 μl)

Desmin

Synonyms:

DES, CSM1, CSM2, LGMD2R, desmin

Immunogen:

Recombinant protein corresponding to Mouse desmin

Uniprot ID:

P31001

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cytoplasm,Cell membrane

Predicted MW. / Observed MW.

54 kDa / 55 kDa

WB

Mouse, Rat

1: 500-1: 2000

heart

IHC/IF

Mouse, Rat

1: 500-1: 1000

heart

WB analysis of Desmin(GB15075).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

IHC analysis of Desmin(GB15075).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Desmin(GB15075).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.