Anti-E Cadherin Rabbit pAb (100 μl)

Anti-E Cadherin Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB11082-100
Availability: In Stock
$259.00
-+
Reactivity: H,M,R & H,M,R    
Applications: WB & IHC/IF    
Host Species: Rabbit    
Clonality: Polyclonal    
Full Name:

E Cadherin rabbit polyclonal antibody

Gene Name:

Cadherin-1

Synonyms:

CDH1, Arc-1, CD324, CDHE, ECAD, LCAM, UVO, cadherin 1, Epithelial Cadherin, E-Cadherin, CAM 120/80

Immunogen:

KLH conjugated Synthetic peptide corresponding to Mouse E Cadherin

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cell membrane, Cytoplasm

Predicted MW.

98 kDa

Observed MW.

135 kDa

reactivity (External validation)

Pelodiscus sinensis




Product Usage Information

WB

Human, Mouse, Rat

1: 1000-1: 2000

spleen, lung, kidney

IHC/IF

Human, Mouse, Rat

1: 200-1: 1500

colon, liver, prostate



Background


Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.




Images

01 GB11082_E-cadherin_5126_WB-1.jpg

WB analysis of E-cadherin (GB11082).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

02 GB11082_E-cadherin_5126_WB-2.jpg

WB analysis of E-cadherin (GB11082).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

03 GB11082_E-cadherin_032_3216060701 H liver d1000_40.0x_IHC-3.jpg


IHC analysis of E-cadherin (GB11082).

Sample: Human liver (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

04 GB11082_E-cadherin_032_3216060701 H liver d500_35.0x_IHC.jpg


IHC analysis of E-cadherin (GB11082).

Sample: Human liver (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

05 GB11082_E-cadherin_032_3216060701 H prostate d500_40.0x_IHC.jpg

IHC analysis of E-cadherin (GB11082).

Sample: Human prostate (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of E-cadherin (GB11082).

Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of E-cadherin (GB11082).

Sample: Mouse stomach (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.


IHC analysis of E-cadherin (GB11082).

Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IF analysis of E-cadherin (GB11082).

Sample: Mouse colon (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1900, 4°C overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

IF analysis of E-cadherin (GB11082).

Sample: Mouse uterus (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1900, 4°C overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

IF analysis of E-cadherin (GB11082).

Sample: Rat stomach (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1900, 4°C overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.




Storage

StorageStore at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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