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| Reactivity: | M,R & M,R |
| Applications: | IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | EEA1 rabbit polyclonal |
Gene Name: | Early endosome antigen 1 |
Synonyms: | Eea1, EEA1, MST105, MSTP105, ZFYVE2, Early endosome antigen 1 |
Immunogen: | Recombinant protein corresponding to Mouse EEA1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Uniprot ID: | Q8BL66 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 2000-1: 5000 | colon |
IF | Mouse, Rat | 1: 1000-1: 1500 | colon |
Background
Early endosomes are cytoplasmic compartments that function in receiving and sorting endocytosed proteins for vesicular transport. EEA1 (early endosome antigen 1) is a peripheral membrane protein that co-localizes with the transferrin receptor and Rab5 on early endosomes. EEA1 contains a calmodulin-binding IQ motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. EEA1 has sequence homology to several yeast proteins that have been implicated in membrane trafficking, including Vps27, Fab1 and Vac1. Evidence suggests a possible role for EEA1 in mediating the regulatory effects of 3'-phosphoinositides on membrane trafficking.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse colon using EEA1 (GB11727) at dilution of 1: 2500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
