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Recombinant Anti-EGFP Rabbit mAb (100 μl)
| Reactivity: | |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Enhanced GFP |
Synonyms: | Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP |
Immunogen: | Recombinant protein corresponding to EGFP(enhanced green fluorescent protein) |
Isotype: | lgG |
Purity: | Affinity purification |
Product Usage Information
WB | Species independent | 1: 1000-1: 2000 | cells/ tissues with eGFP |
IHC/IF | Species independent | 1: 500-1: 1000 | cells/ tissues with eGFP |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.
Images
| WB analysis of eGFP (GB15603). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001)inTBST,RT,1h. Primary antibody:1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of eGFP (GB15603)red. Sample: Transfected 293F cells, 4% PFA (G1101) 20min. Antigen retrieval: Permeabilization Solution（G1204），RT, 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit lgG (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
