Your shopping cart is empty!
| Reactivity: | |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Enhanced GFP |
Synonyms: | Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP |
Immunogen: | Recombinant protein corresponding to EGFP( enhanced green fluorescent protein) |
Uniprot ID: | |
Isotype: | IgG |
Purity: | Affinity purification |
Applications
WB | Species independent | 1: 500-1: 1000 | cells/ tissues with eGFP |
ICC/IF | Species independent | 1: 500-1: 1000 | cells/ tissues with eGFP |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.
Images
| Western blot analysis of eGFP (GB11602) at dilution of 1: 500 |
 | Immunocytochemistry analysis of human 293T cells using eGFP (GB11602) (green) at dilution of 1: 1000. Anti-eGFP ( red ) / DAPI nuclear stain ( blue ) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
