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Anti-EIF2S1 Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | EIF2S1 rabbit polyclonal |
Gene Name: | Eukaryotic translation initiation factor 2 subunit 1 |
Synonyms: | EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, eukaryotic translation initiation factor 2 subunit alpha |
Immunogen: | Recombinant protein corresponding to Mouse EIF2S1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 36 kDa |
Observed MW. | 38 kDa |
Subcellular location: | Cytoplasmic granule |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, lung, kidney |
IHC | Mouse, Rat | 1: 500-1: 1500 | brain, pancreas |
IF | Mouse, Rat | 1: 500-1: 1000 | brain, pancreas |
Background
Eukaryotic translation initiation factor 2 subunit 1 (eIF2α) is a protein that in humans is encoded by the EIF2S1 gene. The protein encoded by this gene is the alpha (α) subunit of the translation initiation factor eIF2 complex which catalyzes an early regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA (Met-tRNAiMet) to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (α, 36 kD, this article), beta (β, 38 kD), and gamma (γ, 52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2α.
Images
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Western blot analysis of EIF2S1 (GB11544) at dilution of 1: 1000. Lane 1: Hela cell lysate Lane 2: HCT116 cell lysate Lane 3: Jurkat cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: Mouse lung tissue lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat spleen tissue lysate Lane 8: Rat lung tissue lysate Lane 9: Rat kidney tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded mouse brain using EIF2S1 (GB11544) at dilution of 1: 500 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using EIF2S1 (GB11544) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
