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| Reactivity: | H,M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | ER membrane protein complex subunit 4 |
Synonyms: | EMC4, ER membrane protein complex subunit 4, PIG17, TMEM85, transmembrane protein 85 |
Immunogen: | Recombinant protein corresponding to Mouse EMC4 |
Uniprot ID: | Q9CZX9 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 20 kDa / 17 kDa |
Product Usage Information
WB | Human, Mouse | 1: 300- 1: 600 | liver, brain |
Background
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.
Images
| Western blot analysis of EMC4 (GB114185) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
