Reactivity: | H,M,R & H,M |
Applications: | WB & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Protein disulfide-isomerase A4 |
Synonyms: | Endoplasmic reticulum resident protein 72, ER protein 72, ERp-72, Cai, Erp72, Pdia4, EPR70, PDIR |
Immunogen: | Recombinant protein corresponding to Mouse ERp72 |
Uniprot ID: | P08003 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 72 kDa / 72 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, lung, brain |
ICC/IF | Human, Mouse | 1: 50-1: 200 | HeLa |
Background
ERP72 is also named as ERP70, PDIA4 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC. The full length protein has a signal peptide with 20 amino acid.
Images
Western blot analysis of ERp72 (GB111846) at dilution of 1: 500 | |
Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using ERp72(GB111846) at dilution of 1: 50-1: 200 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |