Reactivity: | H,M,R & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Ephrin B3 rabbit polyclonal antibody |
Gene Name: | Ephrin-B3 |
Synonyms: | Efnb3, EFL6, LERK8, EPLG8, EPH related receptor transmembrane ligand ELKL3 |
Immunogen: | Recombinant protein corresponding to Mouse Ephrin B3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. | 36 kDa |
Observed MW. | 45 kDa |
Uniprot ID: | Q15768, O35393 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, placenta |
IHC/IF | Human, Mouse, Rat | 1: 600-1: 1800 | brain, placenta, substantia nigra |
Background
May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.Tissue specificity: Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Images
Western blot analysis of Efnb3 (GB111999) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded human brain using Efnb3 (GB111999) at dilution of 1: 600 (250x lens) | |
Immunohistochemistry of paraffin embedded human placenta using Efnb3 (GB111999) at dilution of 1: 600 (250x lens) | |
Immunohistochemistry of paraffin embedded rat substantia nigra using EFNB3 (GB111999) at dilution of 1: 1200 (350x lens) | |
Immunofluorescence of paraffin embedded mouse brain using Enfb3 (GB111999) at dilution of 1: 600 (250x lens) | |
Immunofluorescence of paraffin embedded rat brain using Enfb3 (GB111999) at dilution of 1: 600 (250x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |