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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | FIH1 rabbit polyclonal antibody |
Gene Name: | Hypoxia-inducible factor 1-alpha inhibitor |
Synonyms: | FIH 1, FIH-1 , FIH1, HIF1AN, Hypoxia inducible factor 1 alpha inhibitor, Hypoxia inducible factor 1 alpha subunit inhibitor |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse FIH1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. | 40 kDa |
Observed MW. | 43 kDa |
Uniprot ID: | Q8BLR9, Q9NWT6 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, heart, muscle |
IHC/IF | Human, Mouse, Rat | 1: 1500-1: 6000 | brain, colon, colon cancer, gastric cancer |
Background
HHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
Images
 | Western blot analysis of FIH-1 (GB11843) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded human colon cancer using FIH-1 (GB11843) at dilution of 1: 2000 (400x lens) |
 | Immunohistochemistry of paraffin embedded human colon using FIH-1 (GB11843) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded human gastric cancer using FIH-1 (GB11843) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using FIH-1 (GB11843) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using FIH-1 (GB11843) at dilution of 1: 2000 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
