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| Reactivity: | H & H,M |
| Applications: | WB & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | FTO rabbit polyclonal |
Gene Name: | Alpha-ketoglutarate-dependent dioxygenase FTO |
Synonyms: | Fat mass and obesity-associated protein, Protein fatso, m6A(m)-demethylase FTO, Kiaa1752, FTO |
Immunogen: | Recombinant protein corresponding to Mouse FTO |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 58 kDa |
Observed MW. | 58 kDa |
Uniprot ID: |
Applications
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | 293T, Hela, SH-SY5Y |
IF | Human, Mouse | 1: 1500-1: 3000 | stomach, cerebellum, placenta |
Background
FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.
Images
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Western blot analysis of FTO (GB111359) at dilution of 1: 1000 Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lane 3: SH-SY5Y cell lysate |
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Immunofluorescent analysis of paraformaldehyde-fixed human stomach using FTO (GB111359) at dilution of 1: 1500 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse cerebellum using FTO (GB111359) at dilution of 1: 1500 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse placenta using FTO (GB111359) at dilution of 1: 1500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
