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Recombinant Anti-GAPDH Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal ) |
| Gene Name: | Glyceraldehyde-3-phosphate dehydrogenase |
Synonyms: | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
Immunogen: | KLH conjugated synthetic peptide corresponding to Mouse GAPDH |
Uniprot ID: | P16858 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus, Membrane |
Predicted MW. / Observed MW. | 36 kDa / 36 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 3000-1: 5000 | heart, lung, liver, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 3000 | breast cancer, esophageal cancer, kidney |
ICC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | HeLa |
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
Images
| WB analysis of GAPDH (GB15002). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001)inTBST,RT,1h. Primaryantibody:1: 5000,4°C overnight. Secondaryantibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 5000, RT, 1h. |
| IHC analysis of GAPDH (GB15002). Sample: Human breast carcinoma (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of GAPDH (GB15002). Sample: Human esophageal cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of GAPDH (GB15002). Sample: Mouse kindey (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of GAPDH (GB15002). Sample: Rat kindey (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of GAPDH (GB15002). Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| IHC analysis of GAPDH (GB15002). Sample: Rat spinal cord (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
| ICC analysis of GAPDH(GB15002) red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody：Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
