Your shopping cart is empty!
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | GCLC rabbit polyclonal antibody |
Gene Name: | Glutamate--cysteine ligase catalytic subunit |
Synonyms: | Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC |
Immunogen: | Recombinant protein corresponding to Mouse GCLC |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 73 kDa |
Observed MW. | 73 kDa |
Uniprot ID: | P97494 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 2000-1: 5000 | kidney, liver, lung |
IHC/IF | Mouse, Rat | 1: 1000-1: 2000 | kidney, ovary, esophagus |
Background
GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).
Images
| Western blot analysis of GCLC (GB113956) at dilution of 1: 2000 |
| Immunohistochemistry of paraffin embedded mouse kidney using GCLC (GB113956) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse ovary using GCLC (GB113956) at dilution of 1: 700 (400x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using GCLC (GB113956) at dilution of 1: 1000 (200x lens) |
| Immunohistochemistry of paraffin embedded rat ovary using GCLC (GB113956) at dilution of 1: 700 (500x lens) |
| Immunofluorescence of paraffin embedded mouse esophagus using GCLC (GB113956) at dilution of 1: 1000 (400x lens) |
| Immunofluorescence of paraffin embedded embedded mouse kidney using GCLC (GB113956) at dilution of 1: 1000 (400x lens) |
| Immunofluorescence of paraffin embedded embedded rat kidney using GCLC (GB113956) at dilution of 1: 1000 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
