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Anti-GDF 5 Rabbit pAb (100 μl)
| Reactivity: | H,M & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Growth/differentiation factor 5 |
Synonyms: | GDF-5, Bone morphogenetic protein 14, BMP-14, Gdf5, Bmp14, Bp, Gdf-5 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse GDF 5 |
Uniprot ID: | P43027 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Secreted |
Predicted MW. / Observed MW. | 55 kDa / 55 kDa |
Product Usage Information
WB | Human, Mouse | 1: 500-1: 1000 | NIH3T3, U87-MG |
IHC/IF | Human, Rat | 1: 200-1: 400 | colon cancer, endometrial cancer |
Background
Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction . Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG . Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 .
Images
| Western blot analysis of GDF5 (GB112138) at dilution of 1: 1000 |
| Immunofluorescence analysis of paraffin embedded human colon cancer using GDF5 (GB112138) at dilution of 1: 200 |
| Immunofluorescence analysis of paraffin embedded human endometrial cancer using GDF5 (GB112138) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles.|
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
