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Recombinant Anti-GRP78 BiP Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Endoplasmic reticulum chaperone BiP |
Synonyms: | HSPA5, BIP, GRP78, HEL-S-89n, MIF2, Binding immunoglobulin protein, heat shock protein family A (Hsp70) member 5 |
Immunogen: | Recombinant protein corresponding to Mouse GRP78 BiP |
Uniprot ID: | P20029 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum lumen, Melanosome, Cytoplasm |
Predicted MW. / Observed MW. | 78 kDa / 78 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, colon, kidney |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | kidney, testis |
Background
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Images
| WB analysis of GRP78 BiP(GB15098). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of GRP78 BiP(GB15098). Sample: Human kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of GRP78 BiP(GB15098). Sample: Human liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of GRP78 BiP(GB15098). Sample: Mouse liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of GRP78 BiP(GB15098). Sample: Mouse testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of GRP78 BiP(GB15098). Sample: Rat liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of GRP78 BiP(GB15098). Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
