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Anti-GST Tag Mouse mAb (100 μl)
| Reactivity: | |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | GST Tag fusion protein |
Synonyms: | Glutathione S-Transferase, GST, gst tag |
Immunogen: | Recombinant GST protein |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Tag(All species) | 1: 500-1: 1000 | GST-tag fusion protein |
Special note
Epitope labeling has been proved to be an effective method that can be performed on target proteins. Tag antibody can be used in a variety of applications, including ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB and so on.
Background
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
Images
| Western blot analysis of GST (GB12085) at dilution of 1: 1000 Lane 1: The GST protein Lane 2: The GST protein Lane 3: The GST protein Lane 4: The His-Tag fusion protein Lane 5: The His-Tag fusion protein Lane 6: The His-Tag fusion protein |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
